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FavorPrep™ Endotoxin Free Plasmid DNA Extraction Kit Midi

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FavorPrep™ Endotoxin Free Plasmid DNA Extraction Midi Kit

PreparationsCatalog number
2FAPDE 000-Midi-EF
10FAPDE 002-EF-1
25FAPDE 002-EF
INFORMATION

    DESCRIPTION

    he FavorPrep™ Endotoxin Free Plasmid Midi Kit is designed for the isolation of endotoxin-free purified plasmid DNA with anion-exchange technology. This kit uses a gravity-flow column that increases efficiency of DNA-binding capacity. PTR Buffer washes away the endotoxins in just one step. This kit is designed for the convenient, easy, and efficient extraction of pure plasmid DNA and makes the endotoxins less than 0.05 EU/µg DNA that is suitable for the transfection of cultured cells.

    PERFORMANCE DATA

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    SPECIFICATIONS

    Format/Principle: Anion-exchange chromatography (Gravity-flow column)

    Lysate Clarification: Centrifugation

    Sample Size: 60~120 ml of bacterial for high/low-copy number plasmids.

    Size of Plasmid or Constructs: 3 kbp~150 kbp

    Column Binding Capacity: 650 µg DNA/Column

    COMPONENTS AND STORAGE

    ComponentStorage
    PM1 BufferStored at room temperatures between 15~25°C.
    PM2 Buffer
    PM3 Buffer
    PEQ Buffer
    PW Buffer
    PEL Buffer
    PTR Buffer
    PM Midi Columns
    RNase A SolutionStored at -20°C upon receipt.

    PROTOCOL OVERVIEW

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