he FavorPrep™ Endotoxin Free Plasmid Midi Kit is designed for the isolation of endotoxin-free purified plasmid DNA with anion-exchange technology. This kit uses a gravity-flow column that increases efficiency of DNA-binding capacity. PTR Buffer washes away the endotoxins in just one step. This kit is designed for the convenient, easy, and efficient extraction of pure plasmid DNA and makes the endotoxins less than 0.05 EU/µg DNA that is suitable for the transfection of cultured cells.
Format/Principle: Anion-exchange chromatography (Gravity-flow column)
Lysate Clarification: Centrifugation
Sample Size: 60~120 ml of bacterial for high/low-copy number plasmids.
Size of Plasmid or Constructs: 3 kbp~150 kbp
Column Binding Capacity: ≤650 µg DNA/Column
COMPONENTS AND STORAGE
|Stored at room temperatures between 15~25°C.
|PM Midi Columns
|RNase A Solution
|Stored at -20°C upon receipt.