The FavorPrep™ Plasmid Extraction Mini Kit provides a rapid, phenol-free method for the extraction of high-purity plasmid DNA from bacterial culture such as E. coli. Silica membrane based DNA column is utilized in the purification process, and the extraction is carried out in three simple steps: binding/washing/elution. The plasmid DNA bound to the silica membrane, and the contaminants can be removed by wash buffer. The extracted DNA can be used in a variety of applications such as PCR, cloning, sequencing, in vitro transcription, and labeling.
Format/Principle：Mini spin column (silica matrix)
Sample size：1~5 ml of bacterial culture for high/low-copy number plasmids.
Size of plasmid or construct：<15 kb
Operation time：<25 mins
Typical Yield：25~40 μg
Column Binding capacity：≤60 μg DNA/column
Column Applicability：Centrifugation and vacuum
COMPONENTS AND STORAGE
|Stored at room temperatures between 15~ 25°C.
|WF Buffer (Concentrate)
|Wash Buffer (Concentrate)
|RNase A Solution
|Store at -20°C upon receipt.