FAPDE 000-Mini (4 prep)
FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size: 1~ 7 ml culture cells)

Principle: mini spin column (silica matrix)
Sample size: 1 ~ 7 ml
Size of plasmid or construct: < 15 kb
Operation time: < 25 minutes
Typical Yield: 25 ~ 40 µg
Binding capacity: 60 µg/ column
Column applicability: centrifugation and vaccum

Important Notes:
1. Store RNase A at -20 °C upon recipit of kit.
2. Add 0.5 ml of FAPD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the FAPD1 bottle, mix well by vortexing and store the FAPD1 buffer at 4 °C.
3. If precipitates have formed in FAPD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates.
4. Preparation of W1 Buffer and Wash Buffer by adding 96 ~100% ethanol (not provided) for first use.
5. Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.