Name | : | FavorPrep™ Tissue Genomic DNA Extraction Mini Kit (Proteinase K and micropestle provided)(sample: animal tissue; fresh/frozen of whole blood, plasma, buffy coat or cultured cells of animal, fungus or |
Cat NO | : | Cat NO: FATGK 001-2 |
Principle: mini spin column (silica matrix)
Operation time: 30 ~ 60 minutes
Binding capacity: up to 60 µg DNA/ column
Typical yield: 15 ~35 µg/ prep
Column applicability: centrifugation and vaccum
Minimum elution volume: 50 µl
Sample size: < 25 mg animal tissue
1.2 cm mouse tail
< 10 cultured cells
Important Notes:
1. Buffers provided in this system contain irritants. Wear
gloves and lab coat when handling these buffers.
2. Add 1.1 ml sterile ddH2O to Proteinase K tube to
make a 10 mg/ml stock solution. Vortex and make
sure that Proteinase K has been completely
dissolved. Store the stock solution at 4 °C.
3. Add ethanol (96- 100 %) to W1 Buffer and Wash Buffer
when first open.
4. Prepare dry baths or water baths before the
operation: one to 60 °C for step 4 and the other to
70 °C for step 7.
5. Preheat the Elution Buffer to 70 °C for step 13.
6. All centrifuge steps are done at full speed
(~ 18,000 x g) in a microcentrifuge.
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PingTung Agricultural Biotechnology Park
No.37,Nong-Ke Rd., Ping-Tung 908,
Taiwan
Tel: +886-8-762-1829
Fax: +886-8-762-0791
Email: technical@favorgen.com
Email: order@favorgen.com
Shuttleworthstraße 19, A-1210 Vienna
phone: +43 (0)1 292 82 80
fax: +43 (0)1 292 82 80-88
Email: r.englisch@favorgen-europe.com