300 prep
FavorPrep™ Tissue Total RNA Mini Kit (sample: up to100 mg of animal tissue; cultured cells of animal, fungus or bacteria)

Principle: mini spin column (silica matrix)
Operation time: 30 ~ 60 minutes
Binding capacity: up to 100 µg total RNA/ column
Column applicability: centrifugation and vaccum
Minimum elution volume: 40 µl

Important Notes:
1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
3. Caution: ß-mercaptoethanol (ß-Me) is hazardous to human health. perform the procedures involving ß-Me in a chemical fume hood.
4. Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.
5. All centrifuge steps are done at full speed (~18,000 x g) in a microcentrifuge.
6. Prepare RNase-free DNase 1 reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) and make the final concentration of DNase I to 0.5 U/μl.