5 rxn
miRNA Isolation Kit

Principle: mini spin column (silica matrix)
Sample size: up to 1 x10 cultured cells
up to 100 mg tissue Operation time: ~ 30 minutes
Column applicability: centrifugation and vaccum

Favorgen miRNA Isolation Kit is designed for purification of microRNAs (miRNAs) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNAs allows research into biological significant pathways for regulation of gene regulation. The standard protocols for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNAs and other small RNA. In addition, removal of the predominant larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of different sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.

Important Notes:
1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. Store the kit at room temperaturel.
3. Caution: phenol and chlorofrom are hazardous to human health. perform the procedures involving phenol and chlorofrom in a chemical fume hood.
4. Add required volume of ethanol (96- 100 %) to Wash Buffer when first open. Store the solution at room temperature.