INFORMATION
DESCRIPTION
The FavorPrep™ Plasmid Extraction Midi Kit is designed for the isolation of purified plasmid DNA with anion-exchange technology. This kit uses a gravity-flow column that increases efficiency of DNA binding capacity. This kit is designed for the easy and efficient extraction of pure plasmid DNA. The isolated DNA is ready for enzymatic reactions or molecular biology applications.
PERFORMANCE DATA
SPECIFICATIONS
Format/Principle: Anion-exchange chromatography (Gravity-flow column)
Lysate Clarification: Centrifugation
Sample Size: 60~120 ml of bacterial for high/low-copy number plasmids.
Size of Plasmid or Constructs: 3 kbp~150 kbp
Column Binding Capacity: ≤650 µg DNA/Column
COMPONENTS AND STORAGE
| Components | Storage |
|---|---|
| PM1 Buffer | Stored at room temperature between 15~25°C. |
| PM2 Buffer | |
| PM3 Buffer | |
| PEQ Buffer | |
| PW Buffer | |
| PEL Buffer | |
| PM Midi Columns | |
| RNase A Solution | Stored at -20°C upon receipt. |
PROTOCOL OVERVIEW
USER MANUAL
MSDS